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FUNCTIONAL GAIN OF FRUIT NETTED-CRACKING IN AN INTROGRESSION LINE OF TOMATO WITH HIGHER EXPRESSION OF

《农业科学与工程前沿（英文）》 2021年 第8卷 第2期

摘要：

Fruit cracking is a major disorder that affects the integrity of fruit and reduces the commercial value of tomato and other fleshy fruit. Here, we have found a novel fruit ‘netted-cracking’ (FNC) phenotype in tomato introgression line IL4-4 which is present in neither the donor parent (LA0716) nor the receptor parent (M82). An F2 population was generated by crossing IL4-4 with M82 to genetically characterize the FNC gene and this showed that a single dominant gene determined fruit netted-cracking. Further map-based cloning narrowed down the FNC locus to a 230 kb region on chromosome 4. Sequencing and annotation analysis show that FNC(Solyc04 g082540) was the most likely candidate gene. Functional characterization of FNC by overexpressing FNCAC and FNCIL4-4resulted in the fruit netted-cracking phenotype, suggesting that the FNC transcript level results in the functional gain of fruit netted-cracking. These findings were further confirmed by FNC ortholog in netted-cracking pepper and melon, indicating a common regulatory mechanism in different plant species. Furthermore, cytoplasm and nucleus-localized FNC indicates increased expression of genes involved in suberin, lignin, lipid transport and cell wall metabolism. These findings provide novel genetic insights into fruit netted-cracking and offer a way to promote molecular improvement toward cracking resistant cultivars.

 

关键词： fine mapping / fruit netted-cracking / introgression line / transcript level    

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FUNCTIONAL GAIN OF FRUIT NETTED-CRACKING IN AN INTROGRESSION LINE OF TOMATO WITH HIGHER EXPRESSION OF

Chunli ZHANG, Taotao WANG, Jing LI, Danqiu ZHANG, Qingmin XIE, Shoaib MUNIR, Jie YE, Hanxia LI, Yongen LU, Changxian YANG, Bo OUYANG, Yuyang ZHANG, Junhong ZHANG, Zhibiao YE

《农业科学与工程前沿（英文）》   页码 280-291 doi: 10.15302/J-FASE-2020374

摘要： Fruit cracking is a major disorder that affects the integrity of fruit and reduces the commercial value of tomato and other fleshy fruit. Here, we have found a novel fruit ‘netted-cracking’ (FNC) phenotype in tomato introgression line IL4-4 which is present in neither the donor parent (LA0716) nor the receptor parent (M82). An F population was generated by crossing IL4-4 with M82 to genetically characterize the gene and this showed that a single dominant gene determined fruit netted-cracking. Further map-based cloning narrowed down the locus to a 230 kb region on chromosome 4. Sequencing and annotation analysis show that (Solyc04 g082540) was the most likely candidate gene. Functional characterization of by overexpressing and resulted in the fruit netted-cracking phenotype, suggesting that the transcript level results in the functional gain of fruit netted-cracking. These findings were further confirmed by ortholog in netted-cracking pepper and melon, indicating a common regulatory mechanism in different plant species. Furthermore, cytoplasm and nucleus-localized indicates increased expression of genes involved in suberin, lignin, lipid transport and cell wall metabolism. These findings provide novel genetic insights into fruit netted-cracking and offer a way to promote molecular improvement toward cracking resistant cultivars.

关键词： fine mapping     fruit netted-cracking     introgression line     transcript level    

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Feature extraction of hyperspectral images for detecting immature green citrus fruit

Yongjun DING, Won Suk LEE, Minzan LI

《农业科学与工程前沿（英文）》 2018年 第5卷 第4期   页码 475-484 doi: 10.15302/J-FASE-2018241

摘要：

At an early immature growth stage of citrus, a hyperspectral camera of 369–1042 nm was employed to acquire 30 hyperspectral images in order to detect immature green fruit within citrus trees under natural illumination conditions. First, successive projections algorithm (SPA) were implemented to select 677, 804, 563, 962, and 405 nm wavebands and to construct multispectral images from the original hyperspectral images for further processing. Then, histogram threshold segmentation using NDVI of 804 and 677 nm was implemented to remove image backgrounds. Three slope parameters, calculated from the pairs 405 and 563 nm, 563 and 677 nm, and 804 and 962 nm were used to construct a classifier to identify the potential citrus fruit. Then, a marker-controlled watershed segmentation based on wavelet transform was applied to obtain potential fruit areas. Finally, a green fruit detection model was constructed according to Grey Level Co-occurrence Matrix (GLCM) texture features of the independent areas. Three supervised classifiers, logistic regression, random forest and support vector machine (SVM) were developed using texture features. The detection accuracies were 79%, 75%, and 86% for the logistic regression, random forest, and SVM models, respectively. The developed algorithm showed a great potential for identifying immature green citrus for an early yield estimation.

关键词： hyperspectral     green citrus     image processing     fruit detection     precision agriculture     yield mapping    

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Concurrent optimization of structural topology and infill properties with a CBF-based level set method

Long JIANG, Yang GUO, Shikui CHEN, Peng WEI, Na LEI, Xianfeng David GU

《机械工程前沿（英文）》 2019年 第14卷 第2期   页码 171-189 doi: 10.1007/s11465-019-0530-5

摘要： In this paper, a parametric level-set-based topology optimization framework is proposed to concurrently optimize the structural topology at the macroscale and the effective infill properties at the micro/meso scale. The concurrent optimization is achieved by a computational framework combining a new parametric level set approach with mathematical programming. Within the proposed framework, both the structural boundary evolution and the effective infill property optimization can be driven by mathematical programming, which is more advantageous compared with the conventional partial differential equation-driven level set approach. Moreover, the proposed approach will be more efficient in handling nonlinear problems with multiple constraints. Instead of using radial basis functions (RBF), in this paper, we propose to construct a new type of cardinal basis functions (CBF) for the level set function parameterization. The proposed CBF parameterization ensures an explicit impose of the lower and upper bounds of the design variables. This overcomes the intrinsic disadvantage of the conventional RBF-based parametric level set method, where the lower and upper bounds of the design variables oftentimes have to be set by trial and error. A variational distance regularization method is utilized in this research to regularize the level set function to be a desired distance-regularized shape. With the distance information embedded in the level set model, the wrapping boundary layer and the interior infill region can be naturally defined. The isotropic infill achieved via the mesoscale topology optimization is conformally fit into the wrapping boundary layer using the shape-preserving conformal mapping method, which leads to a hierarchical physical structure with optimized overall topology and effective infill properties. The proposed method is expected to provide a timely solution to the increasing demand for multiscale and multifunctional structure design.

关键词： concurrent topology optimization     parametric level set method     cardinal basis function     shell-infill structure design     conformal mapping    

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YIELD AND FRUIT QUALITY OF ALMOND, PEACH AND PLUM UNDER REGULATED DEFICIT IRRIGATION

《农业科学与工程前沿（英文）》 2021年 第8卷 第4期   页码 583-593 doi: 10.15302/J-FASE -2020325

摘要：

The effects of regulated deficit irrigation (RDI) on the performance of almond cv. Tuono, peach cv. JH-Hall and plum cv. Stanley were assessed on the Saiss Plain (NW, Morocco) over three consecutive growing seasons (2011–2013). Irrigation treatments consisted of a control, irrigation applied to fully satisfy crop water requirements (100% ETC), and two RDI treatments, irrigation applied to 75% ETC (RDI-75) and 50% ETC (RDI-50). These three treatments were applied during fruit-growth slowdown periods corresponding to Stages II and III in almond and Stage II in peach and plum. Yield and fruit quality traits were determined. The effect of RDI differed between species. Yield and fruit size were reduced significantly only in peach under RDI-50. Fruit quality improved in this species in the first year of the experiment, with an increase of sugar/acid ratio and polyphenol content. Plum quality also improved but the effects were significant only in the second and third years. Similar results were recorded in almond kernel, but their epidermal grooves were deeper under RDI-50, and this may have affected their commercial value. It is concluded that water can be saved during the fruit-growth slowdown period by up to 25% in peach and 50% in almond and plum with improvements in fruit quality without affecting total yield.

 

关键词： fruit quality / fruit yield / Prunus domestica / Prunus dulcis / Prunus persica / regulated deficit irrigation    

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Recent advances in fruit crop genomics

Qiang XU,Chaoyang LIU,Manosh Kumar BISWAS,Zhiyong PAN,Xiuxin DENG

《农业科学与工程前沿（英文）》 2014年 第1卷 第1期   页码 21-27 doi: 10.15302/J-FASE-2014002

摘要： In recent years, dramatic progress has been made in the genomics of fruit crops. The publication of a dozen fruit crop genomes represents a milestone for both functional genomics and breeding programs in fruit crops. Rapid advances in high-throughput sequencing technology have revolutionized the manner and scale of genomics in fruit crops. Research on fruit crops is encompassing a wide range of biological questions which are unique and cannot be addressed in a model plant such as . This review summarizes recent achievements of research on the genome, transcriptome, proteome, miRNAs and epigenome of fruit crops.

关键词： fruit crops     fruit biology     gene function     genomics    

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Machine vision-based automatic fruit quality detection and grading

《农业科学与工程前沿（英文）》 doi: 10.15302/J-FASE-2023532

摘要：

● A machine vision-based prototype system was developed for fruit grading.

关键词： Computer and machine vision     convolution neural network     deep learning     defective fruit detection     fruit grading     microcontroller    

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RECENT ADVANCES IN THE REGULATION OF CLIMACTERIC FRUIT RIPENING: HORMONE, TRANSCRIPTION FACTOR AND EPIGENETIC

《农业科学与工程前沿（英文）》 2021年 第8卷 第2期

摘要：

Fruit ripening is a complex developmental process made up of genetically programmed physiological and biochemical activities. It culminates in desirable changes in the structural and textural properties and is governed by a complex regulatory network. Much is known about ethylene, one of the most important metabolites promoting the ripening of climacteric fruits. However, the dynamic interplay between phytohormones also plays an important part. Additional regulatory factors such as transcription factors (TFs) and epigenetic modifications also play vital role in the regulation of climacteric fruit ripening. Here, we review and evaluate the complex regulatory network comprising interactions between hormones and the action of TFs and epigenetic modifications during climacteric fruit ripening.

 

关键词： climacteric fruit ripening / phytohormones / TFs / epigenetic modifications    

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High-level expression of recombinant IgG1 by CHO K1 platform

Ningning Xu, Jianfa Ou, Al-Karim (Al) Gilani, Lufang Zhou, Margaret Liu

《化学科学与工程前沿（英文）》 2015年 第9卷 第3期   页码 376-380 doi: 10.1007/s11705-015-1531-5

摘要： The Chinese Hamster Ovary (CHO K1) cell was used to express a targeted anti-cancer monoclonal antibody by optimizing the platform of the construction of production cell line in this study. The adherent CHO K1 was first adapted to suspension culture in chemical defined medium. Then the glutamine synthetase (GS) vector was applied to construct a single plasmid to overexpress a monoclonal antibody IgG1. Post transfection, the production of cell pool was optimized by glutamine-free selection and amplification using various concentrations of methionine sulfoximine. The best cell pool of CHO K1/IgG1 was used to screen the top single clone using the limiting dilution cloning. Finally, a high IgG1 production of 780 mg/L was obtained from a batch culture. This study demonstrated that the construction of high producing cell line, from gene to clone, could be completed within six month and the gene amplification improved protein production greatly.

关键词： Chinese hamster ovary (CHO)     monoclonal antibody     IgG1     amplification     cell line development    

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RECENT ADVANCES IN THE REGULATION OF CLIMACTERIC FRUIT RIPENING: HORMONE, TRANSCRIPTION FACTOR AND EPIGENETIC

Yinglin JI, Mingyang XU, Aide WANG

《农业科学与工程前沿（英文）》   页码 314-334 doi: 10.15302/J-FASE-2021386

摘要： Fruit ripening is a complex developmental process made up of genetically programmed physiological and biochemical activities. It culminates in desirable changes in the structural and textural properties and is governed by a complex regulatory network. Much is known about ethylene, one of the most important metabolites promoting the ripening of climacteric fruits. However, the dynamic interplay between phytohormones also plays an important part. Additional regulatory factors such as transcription factors (TFs) and epigenetic modifications also play vital role in the regulation of climacteric fruit ripening. Here, we review and evaluate the complex regulatory network comprising interactions between hormones and the action of TFs and epigenetic modifications during climacteric fruit ripening.

关键词： climacteric fruit ripening     phytohormones     TFs     epigenetic modifications    

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大秦线开行20 kt级重载组合列车系统集成与创新

耿志修

《中国工程科学》 2008年 第10卷 第3期   页码 31-43

摘要：

大秦线是我国第一条双线重载电气化运煤专线，西起北同蒲线，东至秦皇岛，全长653 km，设计运量为1×108 t/a，于1992年12月通车。为满足国民经济发展的要求，须尽快大幅度提高大秦线的运输能力。铁道部依靠自主创新，首次实现了GSM-R技术与Locotrol技术的结合，800 MHz数据电台与Locotrol技术的结合，单套Locotrol系统与SS4机车的结合，以及2台和谐型大功率机车加可控列尾的创新方式，开发了重载配套装备，通过集成创新，使大秦线开行20 kt级重载组合列车获得成功，年运量由2002年的1×108 t提高到2007年的3×108 t，是原设计能力的3倍。

关键词： 中国铁路     大秦线     20 kt级重载组合列车     集成创新    

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我国果树业生产现状和待研究的问题

束怀瑞

《中国工程科学》 2003年 第5卷 第2期   页码 45-48

摘要：

介绍了我国果树资源及果树产业、科研当前水平；阐述了有关果业发展拟研究的五个问题：（1）果树传统品种组群与现代化生产及专业化；（2）果树发展与生态环境建设；（3）重视果树根系研究；（4）果品加工发展要加速研究与中试，加工产业发展要注意科学进程和大、中、小企业结合；（5）建立果品优质生产工程的技术体系。具体包括：品种组合与专业化，“世界品种”，名特优品种的不断选育，无公害基地的环境保护，根系研究对持续发展和提高产量品质的潜力，营养基因型根系，生根基因，建立质量工程技术体系，果品加工业的逐步进程等。

关键词： 果树资源     品种组合     果树根系     果品加工     果树优质工程技术体系    

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YIELD AND FRUIT QUALITY OF ALMOND, PEACH AND PLUM UNDER REGULATED DEFICIT IRRIGATION

Rachid RAZOUK, Abdellah KAJJI, Anas HAMDANI, Jamal CHARAFI, Lahcen HSSAINI, Said BOUDA

《农业科学与工程前沿（英文）》 2021年 第8卷 第4期   页码 583-593 doi: 10.15302/J-FASE-2020325

摘要： The effects of regulated deficit irrigation (RDI) on the performance of almond cv. Tuono, peach cv. JH-Hall and plum cv. Stanley were assessed on the Saiss Plain (NW, Morocco) over three consecutive growing seasons (2011–2013). Irrigation treatments consisted of a control, irrigation applied to fully satisfy crop water requirements (100% ET ), and two RDI treatments, irrigation applied to 75% ET (RDI-75) and 50% ET (RDI-50). These three treatments were applied during fruit-growth slowdown periods corresponding to Stages II and III in almond and Stage II in peach and plum. Yield and fruit quality traits were determined. The effect of RDI differed between species. Yield and fruit size were reduced significantly only in peach under RDI-50. Fruit quality improved in this species in the first year of the experiment, with an increase of sugar/acid ratio and polyphenol content. Plum quality also improved but the effects were significant only in the second and third years. Similar results were recorded in almond kernel, but their epidermal grooves were deeper under RDI-50, and this may have affected their commercial value. It is concluded that water can be saved during the fruit-growth slowdown period by up to 25% in peach and 50% in almond and plum with improvements in fruit quality without affecting total yield.

关键词： fruit quality     fruit yield     Prunus domestica     Prunus dulcis     Prunus persica     regulated deficit irrigation    

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Collaborative control of fine particles and ozone required in China for health benefit

《环境科学与工程前沿（英文）》 2023年 第17卷 第8期 doi: 10.1007/s11783-023-1692-2

摘要：

● Increased DAAO offsets 3/4 of the decrease of DAAP in 2013–2020.

关键词： Excess deaths     Long-term exposure     Fine particle     Ozone    

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Chemical mapping of cement pastes by using confocal Raman spectroscopy

Fengjuan LIU,Zhihui SUN

《结构与土木工程前沿（英文）》 2016年 第10卷 第2期   页码 168-173 doi: 10.1007/s11709-015-0323-9

摘要： In the present study, chemical mapping on the hydration process of cement paste with water-to-cement ( / ) ratio of 0.60 was implemented by Raman spectroscopy (RS). The RS was applied to study the paste from 12 hours after mixing to 28 days. Cement ingredients and hydration products, including calcium silicates (C S and C S), calcium hydroxide (CH), and ettringite, were quantitatively studied. From the research, it is observed that calcium silicates were consumed gradually with the increased hydration age. Calcium hydroxide increased and tended to cluster in the pore vicinity during hydration. Ettringite was found to form on the surface of the unreacted particles, which was shown as a mixed zone of ettringite and calcium silicates in the maps. It is concluded from the study that chemical mapping was an effective method to assist in visualizing particle dispersion and connection on top of the quantitative analysis.

关键词： chemical mapping     Raman spectroscopy     calcium silicates     calcium hydroxide     ettringite    

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标题 作者 时间 类型 操作

FUNCTIONAL GAIN OF FRUIT NETTED-CRACKING IN AN INTROGRESSION LINE OF TOMATO WITH HIGHER EXPRESSION OF

期刊论文

FUNCTIONAL GAIN OF FRUIT NETTED-CRACKING IN AN INTROGRESSION LINE OF TOMATO WITH HIGHER EXPRESSION OF

Chunli ZHANG, Taotao WANG, Jing LI, Danqiu ZHANG, Qingmin XIE, Shoaib MUNIR, Jie YE, Hanxia LI, Yongen LU, Changxian YANG, Bo OUYANG, Yuyang ZHANG, Junhong ZHANG, Zhibiao YE

期刊论文

Feature extraction of hyperspectral images for detecting immature green citrus fruit

Yongjun DING, Won Suk LEE, Minzan LI

期刊论文

Concurrent optimization of structural topology and infill properties with a CBF-based level set method

Long JIANG, Yang GUO, Shikui CHEN, Peng WEI, Na LEI, Xianfeng David GU

期刊论文

YIELD AND FRUIT QUALITY OF ALMOND, PEACH AND PLUM UNDER REGULATED DEFICIT IRRIGATION

期刊论文

Recent advances in fruit crop genomics

Qiang XU,Chaoyang LIU,Manosh Kumar BISWAS,Zhiyong PAN,Xiuxin DENG

期刊论文

Machine vision-based automatic fruit quality detection and grading

期刊论文

RECENT ADVANCES IN THE REGULATION OF CLIMACTERIC FRUIT RIPENING: HORMONE, TRANSCRIPTION FACTOR AND EPIGENETIC

期刊论文

High-level expression of recombinant IgG1 by CHO K1 platform

Ningning Xu, Jianfa Ou, Al-Karim (Al) Gilani, Lufang Zhou, Margaret Liu

期刊论文

RECENT ADVANCES IN THE REGULATION OF CLIMACTERIC FRUIT RIPENING: HORMONE, TRANSCRIPTION FACTOR AND EPIGENETIC

Yinglin JI, Mingyang XU, Aide WANG

期刊论文

大秦线开行20 kt级重载组合列车系统集成与创新

耿志修

期刊论文

我国果树业生产现状和待研究的问题

束怀瑞

期刊论文

YIELD AND FRUIT QUALITY OF ALMOND, PEACH AND PLUM UNDER REGULATED DEFICIT IRRIGATION

Rachid RAZOUK, Abdellah KAJJI, Anas HAMDANI, Jamal CHARAFI, Lahcen HSSAINI, Said BOUDA

期刊论文

Collaborative control of fine particles and ozone required in China for health benefit

期刊论文

Chemical mapping of cement pastes by using confocal Raman spectroscopy

Fengjuan LIU,Zhihui SUN

期刊论文